Journal: International Journal of Molecular Sciences

Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex

doi: 10.3390/ijms26083538

Figure Lengend Snippet: The impact of burn injury on cortical neuronal activity: c-Fos immunoreactivity (IR) analysis. ( a ) Schematic representation illustrating the time scale of this experiment. ( b ) Schematic drawing of a coronal section of the mouse brain representing the lower limb area of the S1 cortex. ( c ) Representative immunostaining with an antibody against c-Fos (red) was obtained from three optical sections of a coronal slice of a wild-type mouse brain following burn injury. DAPI counterstained cell nuclei for turquoise. After the burn injury, the number of cells exhibiting c-Fos-IR increased in the lower limb area of the S1 cortex, irrespective of the sides. The term “burn injury-contralateral” specifically refers to the right hemisphere of the brain, which is opposite the burn injury site. Scale bar, 220 μm. ( d ) Quantitative analysis of the percentage of c-Fos-IR nuclei in the lower limb area of the S1 cortex following burn injury. The data are presented as mean ± SEM (10–11 slices from 4 animals per group). An asterisk denotes a p -value < 0.05, while a double asterisk indicates a p -value < 0.01, vs. control. ( e ) Quantitative analysis of the percentage of colocalization between c-Fos and various interneuron subtypes in the S1 cortex following burn injury. The data are presented as mean ± SEM (5–11 slices from 2–4 animals per group). * signifies p < 0.05, while ** indicates p < 0.01, vs. control. ( f ) Representative triple immunostaining with antibodies against c-Fos, parvalbumin (Pvalb), and calretinin (CR) from layer 2/3 of the contralateral-sided S1 cortex following burn injury. Arrowhead shows a CR-positive interneuron that expresses c-Fos. Scale bar, 30 μm. ( g ) Representative triple immunostaining with antibodies against c-Fos, calbindin (Calb), and a nuclear marker, DAPI, from layer 2/3 of the contralateral-sided S1 cortex following burn injury. The dotted line indicates the boundary between layer 1 and layer 2/3. Arrowheads show colocalizations of c-Fos and Calb. Scale bar, 30 μm. Abbreviations: WT, wild type; PFA, paraformaldehyde; S1, primary somatosensory cortex; MOp, primary motor cortex; V3, third ventricle; IC, internal capsule; LV, lateral ventricle; CC, corpus callosum.

Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

Techniques: Activity Assay, Immunostaining, Control, Triple Immunostaining, Marker

Journal: International Journal of Molecular Sciences

Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex

doi: 10.3390/ijms26083538

Figure Lengend Snippet: List of the primary antibodies used in the study, including their suppliers, catalog numbers, and dilution factors.

Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

Techniques:

Journal: Cell reports

Article Title: Spinal microcircuits go through multiphasic homeostatic compensations in a mouse model of motoneuron degeneration

doi: 10.1016/j.celrep.2024.115046

Figure Lengend Snippet: (A) Examples of IPSPs (baseline adjusted for representation) to ventral-root-stimulation obtained from motoneurons in the presence of 4 and 2 mM extracellular Ca 2+ , next to respective histogram counts. (B–E) BQA parameters for quantal size, number of release sites, and probability of release for (D) 2 mM and (E) 4 mM extracellular Ca 2+ . (F) Examples of voltage-clamp motoneuron responses to 200-Hz ventral-root stimulation without (left) and with 4 mM Sr 2+ (right), a large ion that extends synaptic release, thus allowing detection of asynchronous IPSCs (aIPSCs) following extracellular stimulation (see zoomed-in window). (G) Estimation plots for of aIPSC amplitude conductance. (H) Examples of P21 mice identified Renshaw cell boutons (GlyT2 + and Calbindin + ) juxtaposed to motoneurons (vAChT), with labeled clusters of GlyRs (GlycineR) for both control (left) and mSOD1 (right) mice. Top row shows motoneuron somata. The boxes in the top row indicate the position of the two boutons highlighted in the bottom row (represented rotated). (I–K) Group data for (I) GlyR area, (J) perimeter, and (K) number per bouton. Estimation plots with all individual values and respective boxplots shown with respective bootstrapped mean difference and bootstrapped Hedges’ g for (B–E) plots and Kernel smooth distribution with respective linear mixed-model (LMM) estimates shown for plots (I–K). Hierarchical bootstrap used for (G) with mean amplitude per motoneuron used in boxplots. See also and and .

Article Snippet: Chicken Calbindin D28K Polyclonal antibody , Invitrogen | Thermo Fisher Scientific , Cat# PA5-143561, RRID:AB_2942790.

Techniques: Labeling, Control

Journal: Cell reports

Article Title: Spinal microcircuits go through multiphasic homeostatic compensations in a mouse model of motoneuron degeneration

doi: 10.1016/j.celrep.2024.115046

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Chicken Calbindin D28K Polyclonal antibody , Invitrogen | Thermo Fisher Scientific , Cat# PA5-143561, RRID:AB_2942790.

Techniques: Software, Microscopy